UNIVERSITY OF COLOGNE

2024

• Racho J, Riemer J. (2024) Approaches for the analysis of redox-dependent protein import into mitochondria of mammalian cells, Academic Press (Book Chapter)
• Riemer J, Sies H (2024) Mitochondrial Hydrogen Peroxide Dynamics. In The Dynamic Nature of Mitochondria, 89-95 (Book Chapter)
• Zimmermann J, Lang L, Calabrese G, Laporte H, Amponsah PS, Michalk C, Sukmann T, Oestreicher J, Tursch A, Peker E, Owusu TNE, Weith M, Roma LP, Deponte M#, Riemer J#, Morgan B# (2025) Tsa1 is the dominant peroxide scavenger and a source of H₂O₂-dependent GSSG production in yeast. Free Radical Biology and Medicine 226:408-420
• Rothemann RA*, Stobbe D*, Hoehne-Wiechmann MN*, Murschall LM, Peker E, Knaup LK, Racho J, Habich M, Gerlich S, Lapacz KJ, Ulrich K, Riemer J# (2024) Interaction with the cysteine-free protein HAX1 expands the substrate specificity and function of MIA40 beyond protein oxidation. FEBS Journal 291(24):5506-5522
• Scherschel M*, Niemeier JO*, Jacobs LJHC, Hoffmann M, Diederich A, Bell C, Höhne P, Raetz S, Kroll JB, Steinbeck J, Lichtenauer S, Multhoff J, Zimmermann J, Sadhanasatish T, Rothemann RA, Grashoff C, Messens J, Ampofo E, Laschke MW, Riemer J, Prates Roma L, Schwarzländer M#, Morgan B# (2024) The NAPstar family of NADP redox state sensors highlights glutathione as the primary mediator of anti-oxidative electron flux. Nature Communications 15, 10704

2023

• Peker E, Weiss K, Song J, Zarges C, Gerlich S, Boehm V, Trifunovic A, Langer T, Gehring NH, Becker T, Riemer J. (2023) A two-step mitochondrial import pathway couples the disulfide relay with matrix complex I biogenesis, J Cell Biol. 222(7):e202210019.
• Svenja Lenhard*, Sarah Gerlich*, Azkia Khan, Saskia Rödl, Jan-Eric Bökenkamp, Esra Peker, Christine Zarges, Janina Faust, Zuzana Storchova, Markus Räschle, Jan Riemer, Johannes M. Herrmann; The Orf9b protein of SARS-CoV-2 modulates mitochondrial protein biogenesis. J Cell Biol  222(10): e202303002.
• Nanadikar MS, Vergel Leon AM, Guo J, van Belle G, Jatho A, Brandner A, Böckmann R, Rhunzu S, Brodesser S, Schmidtendorf M, Lee J, Wu H, Furdui C, Burgoyne J, Bogeski I, Riemer J, Chowdhury A, Rehling P, Bruegmann T, Belousov V, Katschinski D (2023) IDH3γ serves as a redox switch that regulates mitochondrial energy metabolism and contractility in the heart under oxidative stress. Nature Communications 14(1):2123
• Jacobs LJHC, Riemer J (2023) Maintenance of small molecule redox homeostasis in mitochondria. FEBS Lett., 597(2), 205-223
• Calabrese G, Jacobs LJHC, Riemer J (2023) Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells. Book chapter in Methods Mol Biol. 2023;2675:149-165
• Buntenbroich I, Anton V, Perez-Hernandez D, Simões T, Gaedke F, Schauss A, Dittmar G, Riemer J, Escobar-Henriques M. (2023) Docking and stability defects in mitofusin highlight the proteasome as a potential therapeutic target. iScience.26(7):107014

2022

• Hermeling JCW, Herholz M, Baumann L, Cores EC, Zecic A, Hoppe T, Riemer J, Trifunovic A (2022) Mitochondria-originated redox signalling regulates KLF-1 to promote longevity in Caenorhabditis elegans, Redox Biol 58:102533
• Jacobs LJ, Hoehne MN, Riemer J (2022) Measuring Intracellular H₂O₂ in Intact Human Cells Using the Genetically Encoded Fluorescent Sensor HyPer7. Bio-protocol 12 (20)
• Jacobs LJ, Riemer J. (2022) Maintenance of small molecule redox homeostasis in mitochondria. FEBS Lett. doi: 10.1002/1873-3468
• Bolgi O, Silva-Garcia M, Ross B, Pilla E, Kari V, Killisch M, Spitzner M, Stark N, Lenz C, Weiss K, Donzelli L, Gorrell MD, Grade M, Riemer J, Urlaub H, Dobbelstein M, Huber R, Geiss-Friedlander R. (2022) Dipeptidyl peptidase 9 triggers BRCA2 degradation and promotes DNA damage repair. EMBO Rep. e54136
• Salscheider SL*, Gerlich S*, Cabrera-Orefice A*, Peker E, Rothemann RA, Murschall LM, Finger Y, Szczepanowska K, Ahmadi ZA, Guerrero-Castillo S, Erdogan A, Becker M, Ali M, Habich M, Petrungaro C, Burdina N, Schwarz G, Klußmann M, Neundorf I, Stroud DA, Ryan MT, Trifunovic A, Brandt U, Riemer J. (2022) AIFM1 is a component of the mitochondrial disulfide relay that drives complex I assembly through efficient import of NDUFS5. EMBO J. e110784
• Weiss K, Racho J, Riemer J (2022) Compartmentalized disulfide bond formation pathways. Redox Chemistry and Biology of Thiols, 321-340 (Book Chapter)
• Li X, Straub J, Medeiros TC, Mehra C, den Brave F, Peker E, Atanassov I, Stillger K, Michaelis JB, Burbridge E, Adrain C, Münch C, Riemer J, Becker T, Pernas LF (2022) Mitochondria shed their outer membrane in response to infection-induced stress Science 375(6577)_eabi4343
• Hoehne MN, Jacobs LJHC, Lapacz KJ, Calabrese G, Murschall LM, Marker T, Kaul H, Trifunovic A, Morgan B, Fricker M, Belousov VV, Riemer J, (2022) Spatial and temporal control of mitochondrial H2O2 release in intact human cells. EMBO J, e109169
• Gibhardt CS, Riemer J, Bogeski I (2022) Calcium and redox signals at mitochondrial interfaces: A nanoview perspective. Cell Calcium 103:102550

2021

• Murschall LM*, Peker E*, MacVicar T, Langer T, Riemer J (2021) Protein Import Assay into Mitochondria Isolated from Human Cells. Bio-protocol 11 (12), e4057-e4057
• Schlagowski AM, Knöringer K, Morlot S, Sánchez Vicente A, Flohr T, Krämer L, Boos F, Khalid N, Ahmed S, Schramm J, Murschall LM, Haberkant P, Stein F, Riemer J, Westermann B, Braun RJ, Winklhofer KF, Charvin G, Herrmann JM (2021) Increased levels of mitochondrial import factor Mia40 prevent the aggregation of polyQ proteins in the cytosol. EMBO J. e107913
• Bebber CM, Thomas ES, Stroh J, Chen Z, Androulidaki A, Schmitt A, Höhne MN, Stüker L, de Pádua Alves C, Khonsari A, Dammert MA, Parmaksiz F, Tumbrink HL, Beleggia F, Sos ML, Riemer J, George J, Brodesser S, Thomas RK, Reinhardt HC, von Karstedt S (2021) Ferroptosis response segregates small cell lung cancer (SCLC) neuroendocrine subtypes. Nature Communications 12 (1), 1-19
• Peker E, Erdogan AJ, Volkov AN, Riemer J (2021) Erv1 and Cytochrome c Mediate Rapid Electron Transfer via A Collision-Type Interaction. J Mol Biol. 433(15):167045

2020

• Finger Y*, Habich M*, Gerlich S, Urbanczyk S, Logt E, Koch J, Schu L, Lapacz KJ, Ali M, Petrungaro C, Salscheider SL, Pichlo C, Baumann U, Mielenz D, Brachvogel B, Hofmaann K, Riemer J. (2020). Proteasomal degradation induced by DPP9‐mediated processing competes with mitochondrial protein import. EMBO Journal 2020 Aug e103889
• Szczepanowska K, Senft K, Heidler J, Herholz M, Kukat A, Höhne MN, Hofsetz E, Becker C, Kaspar S, Giese H, Zwicker K, Guerrero-Castillo S, Baumann L, Kauppila J, Rumyantseva A, Müller S, Frese CK, Brandt U, Riemer J, Wittig I, Trifunovic A. (2020) A salvage pathway maintains highly functional respiratory complex I. Nat Commun. 2020 Apr 2;11(1):1643.
• Herrmann JM, Riemer J. (2020) Apoptosis inducing factor and mitochondrial NADH dehydrogenases: redox-controlled gear boxes to switch between mitochondrial biogenesis and cell death. Biol Chem. 2020
• Murschall LM, Gerhards A, MacVicar T, Peker E, Hasberg L, Wawra S, Langer T, Riemer J. (2020) The C-terminal region of the oxidoreductase MIA40 stabilizes its cytosolic precursor during mitochondrial import. BMC Biol. 2020 Aug 6;18(1):96.
• Finger Y, Riemer J (2020) Protein import by the mitochondrial disulfide relay in higher eukaryotes. Biol Chem. 2020 May 26;401(6-7):749-763
• Stavru F, Riemer J, Jex A, Sassera D (2020) When bacteria meet mitochondria: The strange case of the tick symbiont Midichloria mitochondrii. Cellular Microbiology 22(4):e13189.

2019

• MacVicar T, Ohba Y, Nolte H, Mayer FC, Tatsuta T, Sprenger HG, Lindner B, Zhao Y, Li J, Bruns C, Krüger M, Habich M, Riemer J, Schwarzer R, Pasparakis M, Henschke S, Brüning JC, Zamboni N, Langer T. (2019) Lipid signalling drives proteolytic rewiring of mitochondria by YME1L.  Nature 575(7782):361-365 (2019)
• Calabrese G, Peker E, Amponsah PS, Hoehne MN, Riemer T, Mai M, Bienert GP, Deponte M, Morgan B, Riemer J. (2019) Hyperoxidation of mitochondrial peroxiredoxin limits H₂O₂-induced cell death in yeast. EMBO J 2019 Aug 7:e101552. doi: 10.15252/embj.2019101552
• Anton V, Buntenbroich I, Schuster R, Babatz F, Simões T, Altin S, Calabrese G, Riemer J, Schauss A, Escobar-Henriques M (2019) Plasticity in salt-bridge allows fusion-competent ubiquitylation of mitofusins and Cdc48 recognition. Life Science Alliance, 2(6). pii: e201900491 (2019
• Habich M, Salscheider SL, Murschall LM, Hoehne MN, Fischer M, Schorn F, Petrungaro C, Ali M, Erdogan AJ, Abou-Eid S, Kashkar H, Dengjel J, Riemer J. (2019) Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell reports 2019 Jan 15;26(3):759-774.e5. doi: 10.1016/j.celrep.2018.12.092.
• Habich M, Riemer J. (2019) Stop wasting protein-Proteasome inhibition to target diseases linked to mitochondrial import. EMBO Mol Med. 2019 May;11(5).

2018

• Meyer AJ, Riemer J, Rouhier N. (2018) Oxidative protein folding: state-of-the-art and current avenues of research in plants. New Phytol. doi: 10.1111/nph.15436
• Habich M, Salscheider SL, Riemer J. (2018) Cysteine residues in mitochondrial intermembrane space proteins: more than just import. British Journal of Pharmacology. doi: 10.1111/bph.14480.
• Erdogan AJ, Ali M, Habich M, Salscheider SL, Schu L, Petrungaro C, Thomas LW, Ashcroft M, Leichert LI, Roma LP, Riemer J. (2018) The mitochondrial oxidoreductase CHCHD4 is present in a semi-oxidized state in vivo. Redox Biol. doi: 10.1016/j.redox.2018.03.014.
• Roma LP, Deponte M, Riemer J, Morgan B. (2018) Mechanisms and Applications of Redox-Sensitive Green Fluorescent Protein-Based Hydrogen Peroxide Probes. Antioxid Redox Signal. 2018 Jan 10. doi: 10.1089/ars.2017.7449

2017

• Mattie S, Riemer J,  Wideman JG, McBride HM. (2017) A new mitofusin topology places the redox-regulated C terminus in the mitochondrial intermembrane space. J Cell Biol Dec 2017, jcb.201611194; DOI: 10.1083/jcb.201611194
• Döring K, Ahmed N, Riemer T, Suresh HG, Vainshtein Y, Habich M, Riemer J, Mayer MP, O'Brien EP, Kramer G, Bukau B. (2017) Profiling Ssb-Nascent Chain Interactions Reveals Principles of Hsp70-Assisted Folding. Cell. 2017 Jul 13;170(2):298-311.e20. doi: 10.1016/j.cell.2017.06.038
• Calabrese G, Morgan B, Riemer J. (2017) Mitochondrial Glutathione: Regulation and Functions. Antioxid Redox Signal. 2017 May 30. doi: 10.1089/ars.2017.7121
• Habich M, Riemer J. (2017) Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells. Methods Mol Biol 2017 ;1567:105-138. doi: 10.1007/978-1-4939-6824-4_8.
• Friederich MW*, Erdogan AJ*, Coughlin CR 2nd, Elos MT, Jiang H, O'Rourke C, Lovell MA, Wartchow E, Gowan K, Chatfield KC, Chick WS, Spector E, Van Hove JL, Riemer J. (2017) Mutations in the accessory subunit NDUFB10 result in isolated complex I deficiency and illustrate the critical role of intermembrane space import for complex I holoenzyme assembly. Hum Mol Genet. 2017 26 (4): 702-716. doi: 10.1093/hmg/ddw431
• Erdogan AJ, Riemer J. (2017) Mitochondrial disulfide relay and its substrates: mechanisms in health and disease. Cell Tissue Res. 2017 Jan;367(1):59-72

2016

• Appenzeller-Herzog C, Bánhegyi G, Bogeski I, Davies KJ, Delaunay-Moisan A, Forman HJ, Görlach A, Kietzmann T, Laurindo F, Margittai E, Meyer AJ, Riemer J, Rützler M, Simmen T, Sitia R, Toledano MB, Touw IP. (2016) Transit of H2O2 across the endoplasmic reticulum membrane is not sluggish. Free Radic Biol Med. 2016 May;94:157-60

2015

• Petrungaro C, Zimmermann KM, Küttner V, Fischer M, Dengjel J, Bogeski I, Riemer J. (2015) The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake. Cell Metab. 2015 Oct 6;22(4):721-33
• Riemer J, Schwarzländer M, Conrad M, Herrmann JM. (2015) Thiol switches in mitochondria: operation and physiological relevance. Biol Chem. 2015 May;396(5):465-82
• Kojer K, Peleh V, Calabrese G, Herrmann JM, Riemer J. (2015) Kinetic control by limiting glutaredoxin amounts enables thiol oxidation in the reducing mitochondrial IMS. Mol Biol Cell. 2015 Jan 15;26(2):195-204

2014

• Petrungaro C, Riemer J. (2014) Mechanisms and physiological impact of the dual localization of mitochondrial intermembrane space proteins. Biochem Soc Trans. 2014 Aug 1;42(4):952-8
• Kojer K, Riemer J. (2014) Balancing oxidative protein folding - the influences of reducing pathways on disulfide bond formation. Biochim Biophys Acta. 2014 Aug;1844(8):1383-90
• Herrmann JM, Riemer J. (2014) Three approaches to one problem: Protein folding in the periplasm, the endoplasmic reticulum and the intermembrane space. Antioxid Redox Signal. 2014 Jul 20;21(3):438-56
• Longen S, Woellhaf MW, Petrungaro C, Riemer J, Herrmann JM. (2014) The Disulfide Relay of the Intermembrane Space Oxidizes the Ribosomal Subunit Mrp10 on Its Transit into the Mitochondrial Matrix. Dev Cell. 2014 Jan 13;28(1):30-42

2013

• Fischer M, Riemer J. (2013) The Mitochondrial Disulfide Relay System: Roles in Oxidative Protein Folding and Beyond. Int J Cell Biol. 2013;2013:742923
• Suzuki Y, Ali M, Fischer M, Riemer J. (2013) Human copper chaperone for superoxide dismutase 1 mediates its own oxidation-dependent import into mitochondria. Nat Commun. 2013 Sep 12;4:2430
• Hoffmann C, Plocharski B, Haferkamp I, Leroch M, Ewald R, Bauwe H, Riemer J, Herrmann JM, Neuhaus HE. (2013) From endoplasmic reticulum to mitochondria: absence of the Arabidopsis ATP antiporter endoplasmic reticulum adenylate transporter1 perturbs photorespiration. Plant Cell. 2013 Jul;25(7):2647-60
• Fischer M, Horn S, Belkacemi A, Kojer K, Petrungaro C, Habich M, Ali M, Küttner V, Bien M, Kauff F, Dengjel J, Herrmann JM, Riemer J. (2013) Protein import and oxidative folding in the mitochondrial intermembrane space of intact mammalian cells. Mol Biol Cell. 2013 Jul;24(14):2160-2170
• Morgan B, Ezeriņa D, Amoako TN, Riemer J, Seedorf M, Dick TP. (2013) Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis. Nat Chem Biol. 2013 Feb;9(2):119-25
• Eckers E, Petrungaro C, Gross D, Riemer J, Hell K, Deponte M. (2013) Divergent molecular evolution of the mitochondrial sulfhydryl:cytochrome c oxidoreductase Erv in opisthokonts and parasitic protists. J Biol Chem. 2013 Jan 25;288(4):2676-88

2012

• Weckbecker D, Longen S, Riemer J, Herrmann JM (2012) Atp23 biogenesis reveals a chaperone-like folding activity of Mia40 in the IMS of mitochondria EMBO J, 31(22):4348-58
• Riemer J, Kins S. (2012) Axonal Transport and Mitochondrial Dysfunction in Alzheimer's Disease. Neurodegener Dis. 2013;12(3):111-24. doi: 10.1159/000342020.
• Kojer K, Bien M, Gangel H, Morgan B, Dick T.P, Riemer J. (2012). Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state. EMBO J. 2012 Jun 15;31(14):3169-82
• Herrmann J, Riemer J (2012). The mitochondrial disulfide relay: redox-regulated protein import into the intermembrane space. J Biol Chem. 2012 Feb 10;287(7):4426-33

2011

• Klöppel C, Suzuki S, Kojer K, Petrungaro C, Longen S, Fiedler S, Keller S, Riemer J (2011). Mia40-dependent oxidation of cysteines in Domain I of Ccs1 controls its distribution between mitochondria and the cytosol. Mol Biol Cell, 22(20):3749-57
• Riemer J, Fischer M, Herrmann JM (2011). Oxidation-driven protein import into mitochondria: Insights and blind spots. Biochim Biophys Acta, 1808(3):981-9.
• Bonn F, Tatsuta T, Petrungaro C, Riemer J, Langer T (2011). Presequence-dependent folding ensures MrpL32 processing by the m-AAA protease in mitochondria. EMBO J, 30(13):2545-56.
• Riemer J, Hansen HG, Appenzeller-Herzog C, Johansson L, Ellgaard L (2011). Identification of the PDI-Family Member ERp90 as an Interaction Partner of ERFAD. PLoS One, 16;6(2):e17037

2010

• Klöppel C, Michels C, Zimmer J, Herrmann JM, Riemer J (2010). In yeast redistribution of Sod1 to the mitochondrial intermembrane space provides protection against respiration derived oxidative stress. Biochem Biophys Res Comm, Dec 3;403(1):114-9.
• Appenzeller-Herzog C, Riemer J, Zito E, Chin KT, Ron D, Spiess M, Ellgaard L (2010). Disulfide production by Ero1α–PDI relay is rapid and effectively regulated. EMBO J, Oct 6;29(19):3318-29.
• Herrmann JM and Riemer J (2010). The Intermembrane Space of Mitochondondria. Antiox Redox Signal , April, 1
• Herrmann JM and Riemer J (2010). Oxidation and reduction of cysteines in the intermembrane space of mitochondria: Multiple facets of redox-control. Antiox Redox Signal, May, 24
• Chao R, Nevin L, Agarwal P, Riemer J, Bai X, Delaney A, Akana M, JimenezLopez N, Bardakjian T, Schneider A, Chassaing N, Schorderet DF, FitzPatrick D, Kwok PY, Ellgaard L, Gould DB, Zhang Y, Malicki J, Baier H, Slavotinek A. (2010) A male with unilateral microphthalmia reveals a role for TMX3 in eye development. PLoS One. May 11;5(5):e10565.
• Bien M, Longen S, Wagener N, Chwalla I, Herrmann JM, Riemer (2010) Mitochondrial disulfide bond formation is driven by intersubunit electron transfer in Erv1 and proofread by glutathione. J. Mol Cell. 37(4):516-28

2009

• Riemer J, Bulleid N, Herrmann JM (2009). Disulfide Formation in the ER and Mitochondria: Two Solutions to a Common Process. Science 324(5932):1284-7
• Longen S, Bien M, Bihlmaier K, Kloeppel C, Kauff F, Hammermeister M, Westermann B, Herrmann JM and Riemer J (2009). Systematic analysis of the twin Cx9C protein family. J Mol Biol. 393(2), 356-68
• Riemer J, Appenzeller-Herzog C, Johansson L, Bodenmiller B, Hartmann-Petersen R and Ellgaard L. (2009). A luminal flavoprotein in endoplasmic reticulum-associated degradation. Proc Nat Acad Sci.106, 14831-14836
• Roth D, Lynes E, Riemer J, Hansen HG, Althaus N, Simmen T, Ellgaard L (2009). A di-arginine motif contributes to the ER-localization of the type I transmembrane ER oxidoreductase TMX4. Biochem J., 425(1):195-205. 
   

2008

• Appenzeller-Herzog C, Riemer J, Christensen B, Sørensen E and Ellgaard L (2008). A Novel Disulfide Switch Mechanism in Ero1alpha Balances ER Oxidation in Human Cells. EMBO J. 27, 2977-87 
   

2006

• Minich T*, Riemer J*, Schulz JB, Wielinga P, Wijnholds J, Dringen R. (2006). The multidrug resistance protein 1 (Mrp1), but not Mrp5, mediates export of glutathione and glutathione disulfide from brain astrocytes. J. Neurochem., 97, 373-84
   

2004

• Riemer J, Hoepken HH, Czerwinska H, Robinson SR, Dringen R. (2004). Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells. Anal. Biochem., 331, 370-5.
   

2002

• Collet JF, Riemer J, Bader MW, Bardwell JC (2002). Reconstitution of a disulfide isomerization system. J. Biol. Chem., 277, 26886-92.
   

2001

• Bader MW, Hiniker A, Regeimbal J, Goldstone D, Haebel PW, Riemer J, Metcalf P, Bardwell JC (2001). Turning a disulfide isomerase into an oxidase: DsbC mutants that imitate DsbA. EMBO J., 20, 1555-62.